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The genome of the influenza A virus family Orthomyxoviridae consists of eight segmented, negative-stranded RNAs, ranging from to 2, nucleotides nt , constituting 13, nt per genome.
The introduction of next-generation sequencing NGS , which delivers high throughput readings  compared to the traditional Sanger dideoxy chain-termination method  , has provided a remarkable cost reduction for microbial genome sequencing. However, a higher error rate due to homopolymeric miscalling and other systematic base-calling biases have been observed in NGS techniques, compared with the Sanger methods  — .
The Sanger technique is regarded to be low throughput and more tedious, due to the requirement of multiple purification or plasmid cloning steps  ,  ,  — .
This protocol, when applied systematically, should hasten the routine genome sequencing work for local influenza surveillance studies.
It was also demonstrated that this protocol is highly applicable for both clinical samples and Madin-Darby canine kidney- MDCK- cultured samples. Fifteen of the 30 were sequenced from cultured isolates of the original clinical sample using a MDCK. Primer Design To ensure the utility of the assay for the sequencing of older as well as future circulating strains, two reference gene sequences were randomly chosen per month from depositions from different countries and dates of collection to available at the NCBI Influenza Virus Resource.
Primer target regions for RT-PCRs for the different gene segments were selected from the conserved regions of the respective aligned gene sequences. The space is enriched by antiques and traditional artefacts and in the back of the pavilion, the villa's chef does magic in the professional kitchen. Villa Avalon 1 also features an air-conditioned media room with a large library of books, comfy sofas and a state-of-the-art TV set for cosy in-villa movie evenings.
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A beautiful beach in Canggu. From a population size of 3,, people, 81 observations are randomly sampled.
The corresponding sample mean is What is the value of the appropriate test statistic for the test of the population mean, and what is the correct decision at the 1 percent significance level? The denominator of the z-test statistic is standard error instead of standard deviation.
If the denominator takes the value of standard deviation 4. Therefore, reject the null hypothesis because the computed test statistics of 2. Gujarati, Essentials of Econometrics, 3rd ed. It becomes more difficult to reject a null hypothesis when it is actually true. The probability of making a type I error increases. The probability of making a type II error decreases.
Answer: a Explanation: Type I error: The rejection of the null hypothesis when it is actually true. Type II error: The failure to reject the null hypothesis when it is actually false. The significance level is the probability of making a type I error.
Decreasing the probability level makes it more difficult to reject the null when it is true. Decreases the probability of making a type I error.